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Although dogs still retain significant anatomical and physiological characteristics from their wolf relatives, selective breeding by humans has introduced significant changes, including variability in body size, conformation, gait, and lifespan

Kleinman, H., & Sosne, G

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Reconstitution introduces: a new pH a new ionic environment a different solvent system If that shift pushes the peptide outside its stability window: charge drops repulsion weakens molecules begin to aggregate This is often described as a solubility cliff The BAC effect Bacteriostatic water is not neutral

J Neurol 259:16681672 Sauerbier A, Jenner P, Todorova A, Chaudhuri KR (2016) Non motor subtypes and Parkinsons disease